UNITED STATES
                       SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                    Form 13F
                               Form 13F COVER PAGE
Report for the Calendar Year or Quarter Ended: 9/30/2010
Check here if Amendment []; Amendment Number:
                                               -------
This Amendment (Check only one.):               [] is a restatement.
                                                [] adds new holdings entries.

Institutional Investment Manager Filing this Report:


Name:      Burke & Herbert Bank & Trust Co.

Address:   PO Box 268 Alexandria


Form 13F File Number:


The institutional investment manager filing this report and the person by whom
it is signed hereby represent that the person signing the report is authorized
to submit it, that all information contained herein is true, correct and
complete, and that it is understood that all required items, statements,
schedules, lists, and tables, are considered integral parts of this form.

Persons Signing this Report on Behalf of Reporting Manager:

Name:   Judith A. Cagnon
Title:  Vice President
Phone:  703-549-6600

Signature,  Place,  and  Date  of  Signing:

/s/ Judith A. Cagnon               Alexandria, VA                     10/4/2010
---------------------------------  ---------------------------------  ----------
[Signature]                        [City, State]                      [Date]



Report  Type  (Check  only  one):

[X]     13F HOLDINGS REPORT. (Check here if all holdings of this reporting
        manager are reported in this report.)

[ ]     13F NOTICE. (Check here if no holdings reported are in this report,
        and all holdings are reported by other reporting manager(s).)

[ ]     13F COMBINATION REPORT. (Check here if a portion of the holdings for
        this reporting manager are reported in this report and a portion are
        reported by other reporting manager(s).)

List of Other Managers Reporting for this Manager:




                              Form 13F SUMMARY PAGE

Report Summary:

Number of Other Included Managers:                    0

Form 13F Information Table Entry Total:             161

Form 13F Information Table Value Total:  $       86,515
                                         --------------
                                         (In Thousands)


List of Other Included Managers:

Provide a numbered list of the name(s) and 13F file number(s) of all
institutional investment managers with respect to which this report is filed,
other than the manager filing this report.
NONE







                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
TRANSOCEAN LTD               REG SHS        H8817H100       28      440 SH       SOLE                    440      0    0
TRANSOCEAN LTD               REG SHS        H8817H100      242    3,763 SH       DEFINED               3,763      0    0
AT&T INC                     COM            00206R102      815   28,485 SH       SOLE                 28,485      0    0
AT&T INC                     COM            00206R102      834   29,161 SH       DEFINED              29,161      0    0
ABBOTT LABS                  COM            2824100        606   11,600 SH       SOLE                 11,600      0    0
ABBOTT LABS                  COM            2824100        604   11,560 SH       DEFINED              11,560      0    0
AIR PRODS & CHEMS INC        COM            9158106         25      300 SH       SOLE                    300      0    0
AIR PRODS & CHEMS INC        COM            9158106        195    2,350 SH       DEFINED               2,350      0    0
ALTRIA GROUP INC             COM            02209S103      478   19,900 SH       SOLE                 19,900      0    0
ALTRIA GROUP INC             COM            02209S103      151    6,300 SH       DEFINED               6,300      0    0
AMERICAN EXPRESS CO          COM            25816109       215    5,116 SH       SOLE                  5,116      0    0
AMERICAN EXPRESS CO          COM            25816109        50    1,200 SH       DEFINED               1,200      0    0
APPLE INC                    COM            37833100       240      845 SH       SOLE                    845      0    0
APPLE INC                    COM            37833100       497    1,750 SH       DEFINED               1,750      0    0
ATLAS PIPELINE PARTNERS LP   UNIT L P INT   49392103       175   10,000 SH       DEFINED              10,000      0    0
AUTOMATIC DATA PROCESSING IN COM            53015103        28      674 SH       SOLE                    674      0    0
AUTOMATIC DATA PROCESSING IN COM            53015103       277    6,600 SH       DEFINED               6,600      0    0
BP PLC                       SPONSORED ADR  55622104       594   14,432 SH       SOLE                 14,432      0    0
BP PLC                       SPONSORED ADR  55622104        77    1,866 SH       DEFINED               1,866      0    0
BANCO BILBAO VIZCAYA ARGENTA SPONSORED ADR  05946K101      189   14,045 SH       SOLE                 14,045      0    0
BANK OF AMERICA CORPORATION  COM            60505104       117    8,960 SH       SOLE                  8,960      0    0
BANK OF AMERICA CORPORATION  COM            60505104       515   39,327 SH       DEFINED              39,327      0    0
BANK OF NEW YORK MELLON CORP COM            64058100         4      150 SH       SOLE                    150      0    0
BANK OF NEW YORK MELLON CORP COM            64058100       487   18,639 SH       DEFINED              18,639      0    0
BERKSHIRE HATHAWAY INC DEL   CL A           84670108       374        3 SH       SOLE                      3      0    0
BERKSHIRE HATHAWAY INC DEL   CL A           84670108       249        2 SH       DEFINED                   2      0    0
BERKSHIRE HATHAWAY INC DEL   CL B NEW       84670702        87    1,055 SH       SOLE                  1,055      0    0
BERKSHIRE HATHAWAY INC DEL   CL B NEW       84670702       249    3,009 SH       DEFINED               3,009      0    0
BLACKROCK INC                COM            09247X101       94      550 SH       SOLE                    550      0    0
BLACKROCK INC                COM            09247X101      162      950 SH       DEFINED                 950      0    0
BOEING CO                    COM            97023105       186    2,802 SH       SOLE                  2,802      0    0
BOEING CO                    COM            97023105        33      500 SH       DEFINED                 500      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      269    9,926 SH       SOLE                  9,926      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      163    6,020 SH       DEFINED               6,020      0    0
BURKE&HERBERT B&T ALEXANDRIA COM            121331102   14,229    6,629 SH       SOLE                  6,629      0    0
BURKE&HERBERT B&T ALEXANDRIA COM            121331102    1,758      819 SH       DEFINED                 819      0    0
CIGNA CORP                   COM            125509109      543   15,180 SH       DEFINED              15,180      0    0
CVS CAREMARK CORPORATION     COM            126650100      370   11,750 SH       DEFINED              11,750      0    0
CHEVRON CORP NEW             COM            166764100      869   10,724 SH       SOLE                 10,724      0    0
CHEVRON CORP NEW             COM            166764100    1,821   22,471 SH       DEFINED              22,471      0    0
CISCO SYS INC                COM            17275R102      311   14,199 SH       SOLE                 14,199      0    0
CISCO SYS INC                COM            17275R102      177    8,100 SH       DEFINED               8,100      0    0
CITIGROUP INC                COM            172967101       35    9,003 SH       SOLE                  9,003      0    0
CITIGROUP INC                COM            172967101      347   88,695 SH       DEFINED              88,695      0    0
COCA COLA CO                 COM            191216100      700   11,964 SH       SOLE                 11,964      0    0
COCA COLA CO                 COM            191216100      502    8,575 SH       DEFINED               8,575      0    0
COLGATE PALMOLIVE CO         COM            194162103      253    3,295 SH       SOLE                  3,295      0    0
COLGATE PALMOLIVE CO         COM            194162103      150    1,950 SH       DEFINED               1,950      0    0
CONOCOPHILLIPS               COM            20825C104       33      580 SH       SOLE                    580      0    0
CONOCOPHILLIPS               COM            20825C104      188    3,280 SH       DEFINED               3,280      0    0
DELL INC                     COM            24702R101       71    5,500 SH       SOLE                  5,500      0    0
DELL INC                     COM            24702R101       61    4,700 SH       DEFINED               4,700      0    0
DISNEY WALT CO               COM DISNEY     254687106      260    7,842 SH       SOLE                  7,842      0    0
DISNEY WALT CO               COM DISNEY     254687106      450   13,600 SH       DEFINED              13,600      0    0
DOMINION RES INC VA NEW      COM            25746U109      527   12,068 SH       SOLE                 12,068      0    0
DOMINION RES INC VA NEW      COM            25746U109      409    9,374 SH       DEFINED               9,374      0    0
DU PONT E I DE NEMOURS & CO  COM            263534109      574   12,872 SH       SOLE                 12,872      0    0
DU PONT E I DE NEMOURS & CO  COM            263534109      794   17,801 SH       DEFINED              17,801      0    0
DUKE ENERGY CORP NEW         COM            26441C105       35    1,963 SH       SOLE                  1,963      0    0
DUKE ENERGY CORP NEW         COM            26441C105      189   10,700 SH       DEFINED              10,700      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
DUN & BRADSTREET CORP DEL NE COM            2.65E+104      237    3,200 SH       DEFINED               3,200      0    0
EMERSON ELEC CO              COM            291011104      161    3,064 SH       SOLE                  3,064      0    0
EMERSON ELEC CO              COM            291011104       98    1,860 SH       DEFINED               1,860      0    0
EXELON CORP                  COM            30161N101      123    2,882 SH       SOLE                  2,882      0    0
EXELON CORP                  COM            30161N101      247    5,800 SH       DEFINED               5,800      0    0
EXXON MOBIL CORP             COM            30231G102    2,672   43,239 SH       SOLE                 43,239      0    0
EXXON MOBIL CORP             COM            30231G102    4,106   66,446 SH       DEFINED              66,446      0    0
FEDERAL REALTY INVT TR       SH BEN INT NEW 313747206      747    9,150 SH       DEFINED               9,150      0    0
FLUOR CORP NEW               COM            343412102      312    6,300 SH       DEFINED               6,300      0    0
FORTUNE BRANDS INC           COM            349631101      369    7,500 SH       DEFINED               7,500      0    0
FREEPORT-MCMORAN COPPER & GO COM            35671D857      992   11,621 SH       DEFINED              11,621      0    0
FRONTIER COMMUNICATIONS CORP COM            35906A108       40    4,879 SH       SOLE                  4,879      0    0
FRONTIER COMMUNICATIONS CORP COM            35906A108       57    7,007 SH       DEFINED               7,007      0    0
GENERAL ELECTRIC CO          COM            369604103    1,710  105,241 SH       SOLE                105,241      0    0
GENERAL ELECTRIC CO          COM            369604103    1,915  117,873 SH       DEFINED             117,873      0    0
GOOGLE INC                   CL A           38259P508       24       45 SH       SOLE                     45      0    0
GOOGLE INC                   CL A           38259P508      179      340 SH       DEFINED                 340      0    0
HEWLETT PACKARD CO           COM            428236103      867   20,612 SH       SOLE                 20,612      0    0
HEWLETT PACKARD CO           COM            428236103    1,080   25,675 SH       DEFINED              25,675      0    0
HOME DEPOT INC               COM            437076102      139    4,375 SH       SOLE                  4,375      0    0
HOME DEPOT INC               COM            437076102      180    5,683 SH       DEFINED               5,683      0    0
HONEYWELL INTL INC           COM            438516106      231    5,262 SH       SOLE                  5,262      0    0
HONEYWELL INTL INC           COM            438516106      250    5,700 SH       DEFINED               5,700      0    0
INTEL CORP                   COM            458140100      333   17,328 SH       SOLE                 17,328      0    0
INTEL CORP                   COM            458140100      245   12,775 SH       DEFINED              12,775      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      726    5,412 SH       SOLE                  5,412      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      715    5,327 SH       DEFINED               5,327      0    0
ISHARES TR                   BARCLY USAGG B 464287226      257    2,365 SH       SOLE                  2,365      0    0
ISHARES TR                   RUSSELL 3000   464287689      221    3,270 SH       DEFINED               3,270      0    0
JPMORGAN CHASE & CO          COM            46625H100      171    4,505 SH       SOLE                  4,505      0    0
JPMORGAN CHASE & CO          COM            46625H100       92    2,420 SH       DEFINED               2,420      0    0
JOHNSON & JOHNSON            COM            478160104    2,891   46,661 SH       SOLE                 46,661      0    0
JOHNSON & JOHNSON            COM            478160104      561    9,060 SH       DEFINED               9,060      0    0
KIMBERLY CLARK CORP          COM            494368103      278    4,269 SH       SOLE                  4,269      0    0
KIMBERLY CLARK CORP          COM            494368103      189    2,898 SH       DEFINED               2,498      0  400
KRAFT FOODS INC              CL A           50075N104      405   13,117 SH       SOLE                 13,117      0    0
KRAFT FOODS INC              CL A           50075N104       61    1,961 SH       DEFINED               1,961      0    0
LOCKHEED MARTIN CORP         COM            539830109      163    2,280 SH       SOLE                  2,280      0    0
LOCKHEED MARTIN CORP         COM            539830109       43      600 SH       DEFINED                 600      0    0
LOWES COS INC                COM            548661107       75    3,350 SH       SOLE                  3,350      0    0
LOWES COS INC                COM            548661107      325   14,600 SH       DEFINED              14,600      0    0
MANULIFE FINL CORP           COM            56501R106      117    9,288 SH       SOLE                  9,288      0    0
MANULIFE FINL CORP           COM            56501R106      210   16,608 SH       DEFINED              16,608      0    0
MCDONALDS CORP               COM            580135101      254    3,404 SH       SOLE                  3,404      0    0
MCDONALDS CORP               COM            580135101      108    1,450 SH       DEFINED               1,450      0    0
MEDCO HEALTH SOLUTIONS INC   COM            58405U102      247    4,746 SH       SOLE                  4,746      0    0
MEDCO HEALTH SOLUTIONS INC   COM            58405U102       71    1,372 SH       DEFINED               1,372      0    0
MEDTRONIC INC                COM            585055106      243    7,224 SH       SOLE                  7,224      0    0
MEDTRONIC INC                COM            585055106      109    3,250 SH       DEFINED               3,250      0    0
MERCK & CO INC NEW           COM            58933Y105      564   15,330 SH       SOLE                 15,330      0    0
MERCK & CO INC NEW           COM            58933Y105      706   19,180 SH       DEFINED              19,180      0    0
MICROSOFT CORP               COM            594918104      310   12,653 SH       SOLE                 12,653      0    0
MICROSOFT CORP               COM            594918104      761   31,075 SH       DEFINED              31,075      0    0
NEXTERA ENERGY INC           COM            65339F101      268    4,932 SH       SOLE                  4,932      0    0
NEXTERA ENERGY INC           COM            65339F101      171    3,140 SH       DEFINED               3,140      0    0
NORFOLK SOUTHERN CORP        COM            655844108      745   12,519 SH       SOLE                 12,519      0    0
NORFOLK SOUTHERN CORP        COM            655844108      393    6,600 SH       DEFINED               6,600      0    0
ORACLE CORP                  COM            68389X105       93    3,450 SH       SOLE                  3,450      0    0
ORACLE CORP                  COM            68389X105      452   16,830 SH       DEFINED              16,830      0    0
PPG INDS INC                 COM            693506107      222    3,050 SH       SOLE                  3,050      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
PEPSICO INC                  COM            713448108      663    9,975 SH       SOLE                  9,975      0    0
PEPSICO INC                  COM            713448108    1,028   15,473 SH       DEFINED              15,473      0    0
PFIZER INC                   COM            717081103    1,026   59,764 SH       SOLE                 59,764      0    0
PFIZER INC                   COM            717081103    1,349   78,539 SH       DEFINED              78,539      0    0
PHILIP MORRIS INTL INC       COM            718172109    1,092   19,500 SH       SOLE                 19,500      0    0
PHILIP MORRIS INTL INC       COM            718172109      411    7,342 SH       DEFINED               7,342      0    0
PROCTER & GAMBLE CO          COM            742718109      602   10,046 SH       SOLE                 10,046      0    0
PROCTER & GAMBLE CO          COM            742718109      422    7,041 SH       DEFINED               7,041      0    0
PROTECTIVE LIFE CORP         COM            743674103    5,059  232,488 SH       SOLE                232,488      0    0
QWEST COMMUNICATIONS INTL IN COM            749121109        2      361 SH       SOLE                    361      0    0
QWEST COMMUNICATIONS INTL IN COM            749121109       72   11,550 SH       DEFINED              11,550      0    0
REGIONS FINANCIAL CORP NEW   COM            7591EP100      590   81,091 SH       SOLE                 81,091      0    0
REGIONS FINANCIAL CORP NEW   COM            7591EP100       20    2,710 SH       DEFINED               2,710      0    0
ROYAL DUTCH SHELL PLC        SPONS ADR A    780259206      324    5,370 SH       SOLE                  5,370      0    0
SPDR SERIES TRUST            BRCLYS YLD ETF 78464A417       39      979 SH       SOLE                    979      0    0
SPDR SERIES TRUST            BRCLYS YLD ETF 78464A417      267    6,675 SH       DEFINED               6,675      0    0
SARA LEE CORP                COM            803111103      110    8,225 SH       SOLE                  8,225      0    0
SARA LEE CORP                COM            803111103      111    8,300 SH       DEFINED               8,300      0    0
SCHLUMBERGER LTD             COM            806857108      234    3,793 SH       SOLE                  3,793      0    0
SCHLUMBERGER LTD             COM            806857108      486    7,890 SH       DEFINED               7,890      0    0
SPACE PROPULSION SYS INC     COM            846192102        0   16,250 SH       SOLE                 16,250      0    0
TJX COS INC NEW              COM            872540109      232    5,200 SH       DEFINED               5,200      0    0
TARGET CORP                  COM            8.76E+110       71    1,326 SH       SOLE                  1,326      0    0
TARGET CORP                  COM            8.76E+110      278    5,210 SH       DEFINED               5,210      0    0
TEXAS INSTRS INC             COM            882508104      145    5,342 SH       SOLE                  5,342      0    0
TEXAS INSTRS INC             COM            882508104      206    7,607 SH       DEFINED               7,607      0    0
3M CO                        COM            88579Y101      591    6,813 SH       SOLE                  6,813      0    0
3M CO                        COM            88579Y101      291    3,358 SH       DEFINED               3,358      0    0
TRAVELERS COMPANIES INC      COM            8.94E+113        2       45 SH       SOLE                     45      0    0
TRAVELERS COMPANIES INC      COM            8.94E+113      230    4,421 SH       DEFINED               4,421      0    0
UNITED TECHNOLOGIES CORP     COM            913017109      237    3,330 SH       SOLE                  3,330      0    0
UNITED TECHNOLOGIES CORP     COM            913017109      652    9,155 SH       DEFINED               9,155      0    0
UNITEDHEALTH GROUP INC       COM            91324P102      200    5,700 SH       SOLE                  5,700      0    0
UNITEDHEALTH GROUP INC       COM            91324P102       28      800 SH       DEFINED                 800      0    0
VERIZON COMMUNICATIONS INC   COM            92343V104      660   20,237 SH       SOLE                 20,237      0    0
VERIZON COMMUNICATIONS INC   COM            92343V104      952   29,215 SH       DEFINED              29,215      0    0
WAL MART STORES INC          COM            931142103      968   18,086 SH       SOLE                 18,086      0    0
WAL MART STORES INC          COM            931142103      500    9,350 SH       DEFINED               9,350      0    0
WELLPOINT INC                COM            94973V107      306    5,400 SH       DEFINED               5,400      0    0
WELLS FARGO & CO NEW         COM            949746101      364   14,476 SH       SOLE                 14,476      0    0
WELLS FARGO & CO NEW         COM            949746101      199    7,928 SH       DEFINED               7,928      0    0