SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Schedule 13G Under the Securities Exchange Act of 1934 (Amendment No. 1) Alfacell Corporation (Name of Issuer) Common Stock, $.001 par value per share (Title of Class of Securities) 015404106 (CUSIP Number) May 6, 2005 (Date of Event Which Requires Filing of this Statement) Check the appropriate box to designate the rule pursuant to which this Schedule is filed: |_| Rule 13d-1(b) |X| Rule 13d-1(c) |_| Rule 13d-1(d) Page 2 of 6 CUSIP NO. 015404106 -------------------------------------------------------------------------------- 1. NAME OF REPORTING PERSONS I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS (ENTITIES ONLY) James O. McCash -------------------------------------------------------------------------------- 2. CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP* (a) |_| (b) |_| -------------------------------------------------------------------------------- 3. SEC USE ONLY -------------------------------------------------------------------------------- 4. CITIZENSHIP OR PLACE OF ORGANIZATION Mr. McCash is a citizen of the United States of American and a resident of the State of Michigan. -------------------------------------------------------------------------------- 5. SOLE VOTING POWER 3,652,991(1)(2)(3) (includes 120,000 shares subject to warrants). ----------------------------------------------------------------- NUMBER OF 6. SHARED VOTING POWER SHARES BENEFICIALLY 0 OWNED BY ----------------------------------------------------------------- EACH 7. SOLE DISPOSITIVE POWER REPORTING PERSON 3,652,991(1)(2)(3) (includes 120,000 shares subject to WITH warrants). ----------------------------------------------------------------- 8. SHARED DISPOSITIVE POWER 0 -------------------------------------------------------------------------------- 9. AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 3,652,991(1)(2)(3) (includes 120,000 shares subject to warrants). -------------------------------------------------------------------------------- 10. CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES* |_| -------------------------------------------------------------------------------- 11. PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 10.0% -------------------------------------------------------------------------------- 12. TYPE OF REPORTING PERSON* IN -------------------------------------------------------------------------------- *SEE INSTRUCTIONS BEFORE FILLING OUT! ---------- (1) 3,599,323 of these shares are held by the James O. McCash Trust, for which this Reporting Person is the Trustee. The Reporting Person disclaims beneficial ownership of these shares except to the extent of his pecuniary interest therein, and the inclusion of these shares in this statements shall not be deemed an admission of beneficial ownership of the reported shares for any purpose. (2) On November 12, 2004, a convertible note, held by the James O. McCash Trust, with a principal amount of $100,000, came due and converted into 224,931 shares of common stock which were retained by the Trust and 224,931 shares subject to warrants which were gifted by the Trust to the McCash Family Limited Partnership. Both the 224,931 shares of common stock and the 224,931 shares subject to warrants were reported by the Reporting Persons on the initial Schedule 13G filed September 24, 2004. (3) On May 6, 2005, a convertible note, held by the James O. McCash Trust, with a principal amount of $200,000, came due and converted into 1,199,890 shares of common stock which were retained by the Trust and are included in the amounts being reported by both Reporting Persons on this Schedule 13G and an aggregate total of 1,399,890 shares subject to warrants which were gifted to the McCash Family Limited Partnership and are not included in the amounts being reported by either Reporting Person on this Schedule 13G. Page 3 of 6 CUSIP NO. 015404106 -------------------------------------------------------------------------------- 1. NAME OF REPORTING PERSONS I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS (ENTITIES ONLY) James O. McCash Trust -------------------------------------------------------------------------------- 2. CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP* (a) |_| (b) |_| -------------------------------------------------------------------------------- 3. SEC USE ONLY -------------------------------------------------------------------------------- 4. CITIZENSHIP OR PLACE OF ORGANIZATION The Trust was formed pursuant to the laws of the State of Michigan. -------------------------------------------------------------------------------- 5. SOLE VOTING POWER 3,599,323(2)(3) (includes 120,000 shares subject to warrants). NUMBER OF ----------------------------------------------------------------- SHARES 6. SHARED VOTING POWER BENEFICIALLY OWNED BY 0 EACH ----------------------------------------------------------------- REPORTING 7. SOLE DISPOSITIVE POWER PERSON WITH 3,599,323(2)(3) ----------------------------------------------------------------- 8. SHARED DISPOSITIVE POWER 0 -------------------------------------------------------------------------------- 9. AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 3,599,323(2)(3) (includes 120,000 shares subject to warrants). -------------------------------------------------------------------------------- 10. CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES* |_| -------------------------------------------------------------------------------- 11. PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 9.8% -------------------------------------------------------------------------------- 12. TYPE OF REPORTING PERSON* OO -------------------------------------------------------------------------------- *SEE INSTRUCTIONS BEFORE FILLING OUT! ---------- (2) On November 12, 2004, a convertible note, held by the James O. McCash Trust, with a principal amount of $100,000, came due and converted into 224,931 shares of common stock which were retained by the Trust and 224,931 shares subject to warrants which were gifted by the Trust to the McCash Family Limited Partnership. Both the 224,931 shares of common stock and the 224,931 shares subject to warrants were reported by the Reporting Persons on the initial Schedule 13G filed September 24, 2004. (3) On May 6, 2005, a convertible note, held by the James O. McCash Trust, with a principal amount of $200,000, came due and converted into 1,199,890 shares of common stock which were retained by the Trust and are included in the amounts being reported by both Reporting Persons on this Schedule 13G and an aggregate total of 1,399,890 shares subject to warrants which were gifted to the McCash Family Limited Partnership and are not included in the amounts being reported by either Reporting Person on this Schedule 13G. Page 4 of 6 This Amendment No. 1 amends and supplements the Schedule 13G filed by the Reporting Persons on September 24, 2004, relating to the Common Stock, no par value of Alfacell Corporation, a Delaware corporation. Item 1(a). Name of issuer: Alfacell Corporation Item 1(b). Address of issuer's principal executive offices: 225 Belleville Avenue Bloomfield, NJ 07003 Item 2(a). Names of person filing: James O. McCash Trust and James O. McCash, as trustee Item 2(b). Address of principal business office: N3820 S. Grand Oak Drive, Iron Mountain, MI 49801 Item 2(c). Citizenship: James O. McCash is a citizen of the United States of America, residing in the State of Michigan. Item 2(d). Title of class of securities: Common Stock, $.001 par value per share Item 2(e). CUSIP No.: 015404106 Item 3. If this statement is filed pursuant to Secs. 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a: (a) |_| Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o); (b) |_| Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c); (c) |_| Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c); (d) |_| Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8); (e) |_| An investment adviser in accordance with Sec. 240.13d-1(b)(1)(ii)(E); (f) |_| An employee benefit plan or endowment fund in accordance with Sec. 240.13d-1(b)(1)(ii)(F); (g) |_| A parent holding company or control person in accordance with Sec. 240.13d-1(b)(ii)(G); (h) |_| A savings association as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813); (i) |_| A church plan that is excluded from the definition of an investment company under section 3(c) (14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3); (j) |_| Group, in accordance with Sec. 240.13d-1(b)(1)(ii)(J). Item 4. Ownership The information in Items 5 through 11 on the cover pages on this Schedule 13G is hereby incorporated by reference. Page 5 of 6 (a) Amount beneficially owned: 3,652,991 shares, of which 53,668 shares held by James O. McCash directly and 3,599,323 shares held by the James O. McCash Trust (includes 120,000 shares subject to warrants). (b) Percent of class: James O. McCash: 10.0% and James O. McCash Trust: 9.8% (c) Number of shares as to which the person has: (i) Sole power to vote or to direct the vote: 3,652,991 shares, of which 53,668 shares held by James O. McCash directly and 3,599,323 shares held by the James O. McCash Trust (includes 120,000 shares subject to warrants). (ii) Shared power to vote or to direct the vote: 0 (iii) Sole power to dispose or to direct the disposition of: 3,652,991 shares, of which 53,668 shares held by James O. McCash directly and 3,599,323 shares held by the James O. McCash Trust (includes 120,000 shares subject to warrants). (iv) Shared power to dispose or to direct the disposition of: 0 Item 5. Ownership of 5 percent or Less of a Class: Not applicable Item 6. Ownership of More than 5 Percent on Behalf of Another Person: Not applicable Item 7. Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person: Not applicable Item 8. Identification and Classification of Members of the Group: Not applicable Item 9. Notice of Dissolution of Group: Not applicable Page 6 of 6 Item 10. Certifications The following certification shall be included if the statement is filed pursuant to Sec. 240.13d-1(c): By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having the purpose or effect. SIGNATURE After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct. Dated: May 6, 2005 JAMES O. MCCASH TRUST By: /s/ James O. McCash ------------------------------- Signature James O. McCash, Trustee ------------------------------- Name