As the foundation of all molecular biology research, the quality of extracted nucleic acids is one of the key factors determining the success of downstream experiments. In the development of nucleic acid extraction reagents, in addition to conventional chemical components, small molecular nucleic acids and enzymes can greatly improve extraction efficiency and eliminate interference from non-target substances, thus becoming vital ingredients in nucleic acid extraction.

01 Proteinase K

Overview

Proteinase K is a serine protease with high enzymatic activity and broad substrate specificity. It preferentially hydrolyzes ester bonds and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing and aromatic amino acids, and is commonly used to degrade proteins for short peptide preparation. It possesses the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases. Two calcium ion binding sites around its active center enhance its stability, enabling it to maintain high enzymatic activity under a wide range of conditions.

Usage

1. Genetic diagnostic kits

2. RNA and DNA extraction kits

3. Extract non-protein components from tissues and degrade protein impurities, such as the preparation of DNA vaccines and heparin

4. Isolation of chromosomal DNA for pulsed-field gel electrophoresis

5. Western blotting

6. R&D and mass production of enzymatic glycated albumin reagents for in vitro diagnostics

Product Introduction

02 DNase I

Overview

Deoxyribonuclease I (DNase I) is an endodeoxyribonuclease capable of digesting single-stranded and double-stranded DNA. It recognizes and cleaves phosphodiester bonds, producing monodeoxyribonucleotides or single/double-stranded oligodeoxyribonucleotides with a phosphate group at the 5'-end and a hydroxyl group at the 3'-end.

Usage

DNase I has an extremely wide range of applications, covering almost all fields involving RNA manipulation.

1. High-sensitivity qRT-PCR

2. Next-generation sequencing (NGS): RNA-Seq library construction

3. In vitro transcription experiments

Product Introduction

03 Poly A Carrier RNA

Overview

It is synthesized by polymerizing adenylic acid in vitro with polynucleotide phosphorylase, consisting of a mixture of polyadenylic acids ranging from 100-mer to 10000-mer.

Usage

1. Block surface adsorption Most plastic consumables carry static electricity and tend to adsorb nucleic acids. Poly A saturates these adsorption sites and greatly reduces the loss of target nucleic acids.

2. Inhibit nuclease activity Various nucleases exist in biological samples and experimental environments. Poly A can inactivate trace nucleases during extraction and preservation, improving the yield and stability of target nucleic acids.

3. Promote co-precipitation During alcohol-mediated nucleic acid purification, Poly A can form complexes with target nucleic acids for co-precipitation, which greatly enhances nucleic acid recovery rate.

Product Introduction

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