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seqWell Launches First Assay-ready, 384-well Library Preparation Kit to Enable Ultra-high Throughput Plasmid and Synthetic Construct Sequencing

By: via Business Wire

ExpressPlex™ HT NGS library preparation kit containing 6,144 indices can significantly reduce bottlenecks in plasmid and amplicon sequencing for synthetic biology, gene editing, and protein engineering applications

seqWell, a global provider of genomic library workflow solutions, today announced the launch of their new ExpressPlex HT Library Preparation Kit (ExpressPlex HT), the first commercially available next-generation sequencing (NGS) library preparation kit containing all the required reagents and indices to enable multiplexing up to 6,144 samples in a pre-plated in 384-well format. ExpressPlex HT uses a 90-minute workflow in a single mastermix reaction to streamline NGS library preparation through massive multiplexing, thereby enabling lower cost, high throughput sequencing of plasmids, amplicons and other synthetic constructs used in synthetic biology.

This press release features multimedia. View the full release here: https://www.businesswire.com/news/home/20240318055628/en/

seqWell's ExpressPlex HT 384-well library prep kit is the first commercially available next-generation sequencing library preparation kit enabling multiplexing up to 6,144 samples in a pre-plated 384-well format. (Graphic: Business Wire)

seqWell's ExpressPlex HT 384-well library prep kit is the first commercially available next-generation sequencing library preparation kit enabling multiplexing up to 6,144 samples in a pre-plated 384-well format. (Graphic: Business Wire)

“ExpressPlex HT was a natural step in broadening our portfolio of scalable, multiplex library preparation solutions,” said Jack Leonard, Chief Technology Officer at seqWell. “We took a holistic view of our customers’ workflows to address challenges across their ultra-high throughput plasmid sequencing processes, including reagent dispensing and inventory management. Sample-specific barcoding and library amplification both take place in a single reaction step. ExpressPlex HT has brought significant improvements in workflow efficiency to our early access customers.”

Key features of ExpressPlex HT that bring a new level of scale and productivity to plasmid and amplicon NGS:

  • Only commercially available, ready-to-use 384-well library preparation solution. Reagents and indices are supplied in a pre-plated, 384-well format, eliminating the need for reagent dispensing and the complex supply chain management of barcodes and primers.
  • Streamlined, ultra-high throughput and automated workflow. Massive multiplexing using 6,144 indices and a fully automated, 90-minute library preparation workflow enable 24-hour sample-to-answer turnaround for greater than 6,000 samples.
  • Lower environmental impact. ExpressPlex HT’s pre-plated reagents, workflow simplicity, and massive multiplexing require less than 10% of the tips and plastics required for other commonly used high throughput library prep kits, providing large savings in consumable budget and up to a 90% reduction in consumables waste.

“High-throughput synthetic biology increasingly relies on repeated cycles of DBTL – design, build, test and learn – where sequencing often plays a crucial role,” said Joe Mellor, Co-Founder and Chief Scientific Officer at seqWell. “Today’s synthetic biology experiments frequently involve thousands of nucleic acid-based synthetic samples such as plasmids and amplicons, and the difficulty of streamlining NGS library preparation and sequencing for these samples is a bottleneck. ExpressPlex HT can alleviate that bottleneck and more efficiently develop actionable insights from ultra-high throughput experiments and production-scale sequencing of synthetic constructs.”

seqWell recently presented data for ExpressPlex HT library preparation kits in a poster entitled, “Ultra-High Throughput Sequencing for Synthetic Biology Discovery,” at the Advances in Genome Biology and Technology (AGBT) meeting. To review the data, visit the ExpressPlexHT_AGBT poster.

About seqWell

seqWell creates scalable genomics technologies that simplify workflows and help enable every life scientist to unlock transformative discoveries with sequencing. seqWell offers a range of next-generation library prep products and workflows that allow customers to achieve higher levels of sample multiplexing quickly and easily. Learn more about us at www.seqwell.com or follow us on Linkedin.

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